function interpn Search Results


ruler function  (Medmont International Pty Ltd)
90
Medmont International Pty Ltd ruler function
Ruler Function, supplied by Medmont International Pty Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ruler function - by Bioz Stars, 2026-04
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probiotics  (Ameta International)
90
Ameta International probiotics
Probiotics, supplied by Ameta International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
probiotics - by Bioz Stars, 2026-04
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piecewiselinear polynomial function  (MathWorks Inc)
90
MathWorks Inc piecewiselinear polynomial function
Piecewiselinear Polynomial Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
piecewiselinear polynomial function - by Bioz Stars, 2026-04
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interp2 function  (MathWorks Inc)
90
MathWorks Inc interp2 function
Interp2 Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/interp2 function/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
interp2 function - by Bioz Stars, 2026-04
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fak  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc fak
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Fak, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
fak - by Bioz Stars, 2026-04
95/100 stars
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weighted decay time constant (weighted)  (Huntsman International LLC)
90
Huntsman International LLC weighted decay time constant (weighted)
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Weighted Decay Time Constant (Weighted), supplied by Huntsman International LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
weighted decay time constant (weighted) - by Bioz Stars, 2026-04
90/100 stars
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chromogenic functional methods  (Dade Behring)
90
Dade Behring chromogenic functional methods
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Chromogenic Functional Methods, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
chromogenic functional methods - by Bioz Stars, 2026-04
90/100 stars
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interpolation function interp1  (MathWorks Inc)
90
MathWorks Inc interpolation function interp1
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Interpolation Function Interp1, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
interpolation function interp1 - by Bioz Stars, 2026-04
90/100 stars
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abundance data set  (Seamap International Holdings Pte Ltd)
90
Seamap International Holdings Pte Ltd abundance data set
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Abundance Data Set, supplied by Seamap International Holdings Pte Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/abundance data set/product/Seamap International Holdings Pte Ltd
Average 90 stars, based on 1 article reviews
abundance data set - by Bioz Stars, 2026-04
90/100 stars
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caqdas nvivo  (qsr international)
90
qsr international caqdas nvivo
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Caqdas Nvivo, supplied by qsr international, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caqdas nvivo/product/qsr international
Average 90 stars, based on 1 article reviews
caqdas nvivo - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
interp function  (MathWorks Inc)
90
MathWorks Inc interp function
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
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Huntsman International LLC gaba a receptor
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
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TG2–FN clusters activate β-catenin in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: TG2–FN clusters activate β-catenin in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Article Snippet: Polyclonal ILK (clone 4G9), nonphospho (active) β-catenin at Ser33/37 and Thr41 (clone D13A1), p-FAK Tyr576/577 , FAK (clone D5O7U), GSK-3α/β, and p-GSK-3α/β Ser21/9 (clone 37F11) antibodies were from Cell Signaling Technology; and GAPDH from Biodesign International.

Techniques: Stable Transfection, Transduction, shRNA, Expressing, Membrane, Transfection, Staining, Software, Luciferase, Control, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Knockdown, Binding Assay, Western Blot

Functional TG2 and integrin β1 inhibition disrupts β-catenin signaling in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, and GAPDH in OVCAR-5 cells treated with inhibitory antibodies (Abs) directed against the FN-binding domain of TG2 (clone 4G3) or integrin β1 (clone P5D2) and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios (N = 3; ∗ p < 0.05; ∗∗ p < 0.01). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). C , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A plated on FN for 2 h. D , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). E , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid, treated with 4G3 or P5D2 Abs and/or Wnt-3A, and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). F , real-time PCR for c-Myc in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h (N = 6; ∗∗∗∗ p < 0.0001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: Functional TG2 and integrin β1 inhibition disrupts β-catenin signaling in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, and GAPDH in OVCAR-5 cells treated with inhibitory antibodies (Abs) directed against the FN-binding domain of TG2 (clone 4G3) or integrin β1 (clone P5D2) and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios (N = 3; ∗ p < 0.05; ∗∗ p < 0.01). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). C , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A plated on FN for 2 h. D , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). E , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid, treated with 4G3 or P5D2 Abs and/or Wnt-3A, and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). F , real-time PCR for c-Myc in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h (N = 6; ∗∗∗∗ p < 0.0001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Article Snippet: Polyclonal ILK (clone 4G9), nonphospho (active) β-catenin at Ser33/37 and Thr41 (clone D13A1), p-FAK Tyr576/577 , FAK (clone D5O7U), GSK-3α/β, and p-GSK-3α/β Ser21/9 (clone 37F11) antibodies were from Cell Signaling Technology; and GAPDH from Biodesign International.

Techniques: Functional Assay, Inhibition, Binding Assay, Expressing, Staining, Software, Luciferase, Control, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Western Blot

ILK inhibition blocks β-catenin signaling in OC cells. A and B , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A. Densitometry quantifies non–p-active β-catenin expression levels and p-FAK Tyr576/577 /FAK, p-ILK Ser246 /ILK, and p-GSKα/β Ser21/9 /GSKα/β ratios (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same GAPDH representative WB images are shown in A and B . C and D , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells sh-Ctr or sh-ILK plated on FN for 2 h and treated or not with Wnt-3A. Densitometry quantifies ILK and non–p-active β-catenin expression levels and p-FAK Tyr576/577 /FAK, p-ILK Ser246 /ILK, and p-GSKα/β Ser21/9 /GSKα/β ratios (N = 3; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001). E , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A. F , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). G , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid prior to treatment with cpd-22 or Wnt-3A and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). H , real-time PCR for c-Myc in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A (N = 6; ∗∗∗∗ p < 0.0001). I , OVCAR-5 cells sh-Ctr or sh-ILK were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid prior to treatment with cpd-22 or Wnt-3A and plated on FN for 2 h (N = 6; ∗∗∗ p < 0.01; ∗∗∗ p < 0.001). J , real-time PCR for c-Myc in OVCAR-5 cells stably transduced with scrambled- or ILK-targeting shRNA plated on FN-coated plates for 2 h and treated or not with Wnt-3A (N = 6; ∗∗∗ p < 0.01; ∗∗∗ p < 0.001). cpd-22, compound 22; FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: ILK inhibition blocks β-catenin signaling in OC cells. A and B , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A. Densitometry quantifies non–p-active β-catenin expression levels and p-FAK Tyr576/577 /FAK, p-ILK Ser246 /ILK, and p-GSKα/β Ser21/9 /GSKα/β ratios (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same GAPDH representative WB images are shown in A and B . C and D , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells sh-Ctr or sh-ILK plated on FN for 2 h and treated or not with Wnt-3A. Densitometry quantifies ILK and non–p-active β-catenin expression levels and p-FAK Tyr576/577 /FAK, p-ILK Ser246 /ILK, and p-GSKα/β Ser21/9 /GSKα/β ratios (N = 3; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001). E , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A. F , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). G , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid prior to treatment with cpd-22 or Wnt-3A and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). H , real-time PCR for c-Myc in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A (N = 6; ∗∗∗∗ p < 0.0001). I , OVCAR-5 cells sh-Ctr or sh-ILK were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid prior to treatment with cpd-22 or Wnt-3A and plated on FN for 2 h (N = 6; ∗∗∗ p < 0.01; ∗∗∗ p < 0.001). J , real-time PCR for c-Myc in OVCAR-5 cells stably transduced with scrambled- or ILK-targeting shRNA plated on FN-coated plates for 2 h and treated or not with Wnt-3A (N = 6; ∗∗∗ p < 0.01; ∗∗∗ p < 0.001). cpd-22, compound 22; FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; WB, Western blot.

Article Snippet: Polyclonal ILK (clone 4G9), nonphospho (active) β-catenin at Ser33/37 and Thr41 (clone D13A1), p-FAK Tyr576/577 , FAK (clone D5O7U), GSK-3α/β, and p-GSK-3α/β Ser21/9 (clone 37F11) antibodies were from Cell Signaling Technology; and GAPDH from Biodesign International.

Techniques: Inhibition, Expressing, Membrane, Staining, Software, Luciferase, Control, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Stable Transfection, Transduction, shRNA, Immunofluorescence, Binding Assay, Western Blot